Friday, September 12, 2008

Microscope Lab and Write-up for Unit One


Microscope Background Write-up:
The first official microscope was designed and used in 1595 in Middleburg, Holland. By the year 1660 in England, Robert Hooke had made improvements to the microscope that allowed him to explore plant cells scientifically and break new ground with his discoveries. Later on, Leeuwen, who was a self taught man, gained scientific knowledge from the book written by Robert Hooke entitled, “Micrographia”. Leeuwen was able to apply this learned information toward his own microscopic improvement. The advancements of Leeuwen allowed him the ability to view and describe minuscule bacteria and protozoan life.
Steps to Use a Microscope:
Turn microscope light on by pushing the on/off
Raise rheostat lever to “10”
Choose Slide/place Slide on Stage and hold slide in place with Stage Clips (looking AT)
Turn Ocular Housing to select an Objective Lens(looking AT) 4X Objective Lens chosen (low power to be used when setting up microscope)
Move Slide to be viewed into the circle of light by adjustments of XY Knobs *one knob forward/back *one knob side/side
Raise Stage completely upward by turning Course Focus knob away from you (looking AT)
Adjust Oculars (eye pieces) by turning until only one circle can be seen through eye pieces *typically set at ‘64’ to begin (looking THROUGH)
Use Course Focus knob slowly until slide image appears (looking THROUGH)
Use Fine Focus knob to improve clarity of image focus/XY Controls are used to move item into center of view/iris diaphragm adjusts the brightness (looking THROUGH)
*Once the image is focused at low level Objective, then you can raise the magnification by a higher numbered Objective Lens to see the image in greater minute detail. To do this, simply follow these steps:
Set the Objective Lens on an increased number setting for higher magnification (+)
* I used 40x for my Lab assignment that is posted at the top. Adjust the Iris Diaphragm lever (lighting) by opening the Iris to view the slide image more clearly.
Adjustments may need to be made to the Fine Focusing to achieve sharper, clearer image.

In Conclusion:
The development and use of microscopes has created great opportunities for the scientific community and laymen alike in the world of Microscopy. Complex cell detail can be viewed, studied, and interpreted. The knowledge gained by the use of microscopes has affected science in many ways. The understanding of cellular matter has allowed advancements medically, agriculturally, and scientifically. The invention of the microscope opened up an entire new world that was not possible to explore prior to this invention.
What I Learned:
The simulator was fun to use! I enjoyed how the program was set up because as I went through the steps it would tell me when I had forgotten to do part of the process or failed to make an adjustment to a knob. I enjoyed the various slides that were offered with the simulator and was inspired to check out other sites online to test out my new found abilities and look at items 'microscopically".

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